Journal: Antibiotics

Article Title: Antimicrobial Spectrum of Titroleane™: A New Potent Anti-Infective Agent

doi: 10.3390/antibiotics9070391

Figure Lengend Snippet: Antimicrobial spectrum of Titroleane™ on bacteria and fungi encountered in human and animal health.

Article Snippet: Solution Hemine 25 mg mL −1 (Hemine chloride (H9039, Sigma-Aldrich, Saint-Quentin-Fallavier, France) and Triethanolamine (421631000, ACROS Organics, Antwerp, Belgium)) were used to grow Fusobacterium nucleatum (CIP 101130), Bacteroides fragilis (CIP 105891), Bacteroides ovatus (CIP 103756), Bacteroides thethaiotaomicron (CIP 104207), Bifidobacterium bifidum (CIP 56.7), Bifidobacterium breve (CIP 64.69), Bifidobacterium lactis (CIP 105265), Bifidobacterium longum longum (CIP 64.62T), Bifidobacterium longum infantis (CIP 64.71) and Propionibacterium acnes (ATCC 6919).

Techniques: Bacteria

Journal: Cell host & microbe

Article Title: Resistin-like molecule α provides vitamin A-dependent antimicrobial protection in the skin

doi: 10.1016/j.chom.2019.04.004

Figure Lengend Snippet: (A,B) S. pyogenes (A) or wild-type or staphyloxanthin-deficient (ΔCRTM) Staphylococcus strains (B) were grown to logarithmic phase and applied on a gauze rectangle to the dorsal skin of Retnla−/− or wild-type mice under occlusion for 2 days. CFU were determined in sections of inoculated skin.

Article Snippet: Relative expression values were determined using the comparative Ct (ΔΔCt) method, and transcript abundances were normalized to Gapdh or 18S transcript abundance. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-RELMα Life Span Cat# LS-C104472; RRID:AB_2180030 anti-RETN Life Span Cat# LS-C131843–100; RRID:AB_10834395 anti-RELMα Abcam Cat# ab39626, RRID:AB_777652 Alexa Fluor® 594 secondary antibody Thermo Fisher Scientific Cat# {"type":"entrez-nucleotide","attrs":{"text":"R37119","term_id":"794575","term_text":"R37119"}} R37119 , RRID:AB_2556547 Anti-Actin Sigma-Aldrich Cat# A5060, RRID:AB_476738 Bacterial and Virus Strains Staphylococcus aureus ATCC ATCC 25923 Escherichia coli K-12 ATCC ATC-PTA-7555 Pseudomonas aeruginosa ATCC ATCC 27853 Listeria monocytogenes , EGD-e BD Biosciences BD# 237500 Staphylococcus epidermidis , clinical isolate Hooper Lab N/A Streptococcus pyogenes ATCC ATCC12384 Propionibacterium acnes ATCC ATCC 6919 Staphylococcus aureus (parent strain) Dr. George Liu N/A Staphylococcus aureus (ΔCRTM) Dr. George Liu N/A Citrobacter rodentium , DBS100 ATCC ATCC 51459 Biological Samples Human skin tissue-Control samples UT Southwestern Tissue Bank/PI: Ben Chong M.D.

Techniques:

Journal: Cell host & microbe

Article Title: Resistin-like molecule α provides vitamin A-dependent antimicrobial protection in the skin

doi: 10.1016/j.chom.2019.04.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Relative expression values were determined using the comparative Ct (ΔΔCt) method, and transcript abundances were normalized to Gapdh or 18S transcript abundance. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-RELMα Life Span Cat# LS-C104472; RRID:AB_2180030 anti-RETN Life Span Cat# LS-C131843–100; RRID:AB_10834395 anti-RELMα Abcam Cat# ab39626, RRID:AB_777652 Alexa Fluor® 594 secondary antibody Thermo Fisher Scientific Cat# {"type":"entrez-nucleotide","attrs":{"text":"R37119","term_id":"794575","term_text":"R37119"}} R37119 , RRID:AB_2556547 Anti-Actin Sigma-Aldrich Cat# A5060, RRID:AB_476738 Bacterial and Virus Strains Staphylococcus aureus ATCC ATCC 25923 Escherichia coli K-12 ATCC ATC-PTA-7555 Pseudomonas aeruginosa ATCC ATCC 27853 Listeria monocytogenes , EGD-e BD Biosciences BD# 237500 Staphylococcus epidermidis , clinical isolate Hooper Lab N/A Streptococcus pyogenes ATCC ATCC12384 Propionibacterium acnes ATCC ATCC 6919 Staphylococcus aureus (parent strain) Dr. George Liu N/A Staphylococcus aureus (ΔCRTM) Dr. George Liu N/A Citrobacter rodentium , DBS100 ATCC ATCC 51459 Biological Samples Human skin tissue-Control samples UT Southwestern Tissue Bank/PI: Ben Chong M.D.

Techniques: Virus, Recombinant, Protease Inhibitor, Control, Cream, Sample Prep, Reverse Transcription, Purification, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation, Expressing, Cloning, Plasmid Preparation, Software, Microscopy